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Image Search Results
Journal: Nature Communications
Article Title: Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth
doi: 10.1038/s41467-020-17181-w
Figure Lengend Snippet: a , b NSG mice with subcutaneous implantation of melanoma PDX tumour TM00702 (Control, n = 10; High Gln, n = 9 biologically independent tumours) received control or high glutamine (High Gln) diet and tumours were measured twice weekly ( a ) and tumour weights post mortem are shown in b . c , d NSG mice with subcutaneous implantation of melanoma PDX tumour TM01612 (Control, n = 8; High Gln, n = 8 biologically independent tumours) received control or High Gln diet and tumours were measured twice weekly ( c ) and tumour weights post mortem are shown in d . e , f Glutamine concentration in PDX TM00702 in e and TM01612 in f from serum and tumours were measured ( n = 8 biological replicates). g Cross-sections of PDX_TM00702 (left) and PDX_TM01612 (right) tumours were H&E stained. Scale bar = 100 μm. Arrows indicate regions of necrosis/apoptosis. h Cross-sections of PDX_TM00702 (left) and PDX_TM01612 (right) tumours were Ki67 stained (Control, n = 3; High Gln, n = 3). Scale bar = 100 μm. Data represent means and error bars are s.d. p value was calculated by t test (unpaired, two tailed) except in a , c by Mann–Whitney test (unpaired, two tailed). * p < 0.05, ** p < 0.01.
Article Snippet: For PDX studies, NSG mice were purchased from
Techniques: Control, Concentration Assay, Staining, Two Tailed Test, MANN-WHITNEY
Journal: Nature Communications
Article Title: Dietary glutamine supplementation suppresses epigenetically-activated oncogenic pathways to inhibit melanoma tumour growth
doi: 10.1038/s41467-020-17181-w
Figure Lengend Snippet: a Hierarchical clustering and heat map of differentially expressed genes (DEGs) in PDX_TM00702 tumours from control (Ctrl) or glutamine supplementation (Gln) mice groups ( n = 4, biological replicates). b Pathway enrichment analysis of all DEGs ( p < 0.05 and FDR < 0.05) in Gln versus Ctrl groups with fold change = ±1.5. c Pathway enrichment analysis of DEGs downregulated in Gln versus Ctrl groups with fold change ≥1.5, p < 0.05 and FDR < 0.05. d GSEA analysis in control (Ctrl) versus glutamine supplemented (Gln) tumours. e Pathway enrichment analysis of DEGs upregulated in Gln versus Ctrl groups with fold change <−1.5, p < 0.05 and FDR < 0.05. f GSEA analysis in control (Ctrl) versus glutamine supplemented (Gln) tumours. g , h Melanoma-associated oncogenes expression in PDX_TM00702 in g and PDX_TM01612 in h tumours verified by qPCR ( n = 3, biological replicates performed in triplicates). Data represent means and error bars are s.d. p value calculated by t test (unpaired, two tailed). Source data are provided as a Source data file.
Article Snippet: For PDX studies, NSG mice were purchased from
Techniques: Control, Expressing, Two Tailed Test